Human Kidney Biomarker Array Kit: R&D Systems

This product has been discontinued.

Human Kidney Biomarker Array Ordering Information

Introduction

R&D Systems Proteome Profiler™ Human Kidney Biomarker Array Kit (Catalog # ARY019) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 38 proteins in a single sample. No specialized equipment is necessary.

Simultaneously detect the levels of these cell stress-related proteins in a single sample.
Adiponectin	Fetuin A	Renin
Aminopeptidase N	GRO-alpha/CXCL1	Resistin
Angiotensinogen/Serpin A8	IL-1ra	SCF
Annexin V	IL-6	Serpin A3
beta2-Microglobulin	IL-10	TNF-alpha
Clusterin	TIM-1/KIM-1/HAVCR	TNF RI
CXCL16	Lipocalin-2/NGAL	Trefoil Factor 3
Cyr61/CCN	MCP-1/CCL2	Thrombospondin-1
Cystatin C	MMP-9	TWEAK
DPPIV/CD26	Neprilysin	uPA
EGF	PSA/KLK-3	VCAM-1
EGF R/ErbB1	RAGE	VEGF-A
FABP1/L-FABP	RBP-4

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Urine, cell culture supernatant, and cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any biomarker/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of kidney biomarker bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

4 Array Membranes
4-Well Multi-dish
Array Buffers
Wash Buffer
Detection Antibody Cocktail
Streptavidin-HRP
Chemiluminescent Detection Reagents
Transparency Overlay Template
Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.